Composite
ColiAlert

Part:BBa_K1565001:Design

Designed by: Deborah M Landim Zanforlin   Group: iGEM14_LIKA-CESAR-Brasil   (2014-10-10)


Colorimetric indicator of cell lysis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1468
    Illegal BamHI site found at 1002
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1878
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1152
    Illegal SapI.rc site found at 2891


Design Notes

The endoglucanase gene was added to biobrick wrongly. The biobrick works and should be made ​​without this gene.


Source

It is formed by biobricks already available in the registry.

References